5 Tips about attune flow cytometer You Can Use Today

Information-enriched characterization of cell populations. Morphological information and facts from illustrations or photos can include towards the richness of flow cytometry info. Such as, the determine shows an or else typical apoptosis assay employing Annexin V and PI, introducing mobile imaging to characterize cells in Every single population to reveal morphologically distinctive functions. These insights could not have already been received from multiplex staining by yourself.

Whether or not you’re a newbie or an authority, explore our resources to advance your study and skills in flow cytometry.

(B) RAW 264.seven macrophages in many cases are annotated inaccurately with respect to particle depend because of their complexity and noncircular borders. The quantity of singlets accessible for Evaluation will increase when the user trains and employs a far more precise product.

The award-successful Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing having a higher-velocity brightfield camera for simultaneous high throughput flow cytometry and significant resolution brightfield imaging.

"An image is not quite worthy of one thousand dots, but it really definitely really helps to see pictures. It is great to see photos that correlate with staining. By way of example, AnnexinV-optimistic cells which might be morphologically distinctive than healthy cells, or CD14+ monocytes which might be much larger and even more textured than CD3+/CD4+/CD14- lymphocytes.

Below, a highly skilled user has gated singlets confidently. Soon after assessing the handbook singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate has greater than four% aggregates.

Optimizing gating tactic. Even strong handbook singlet gating is error-prone and remains a subjective selection stage in Nearly all flow cytometry assays. Imaging may be used to verify and change gates to incorporate only one cells of curiosity.

Regular graphic high quality even at superior throughput Acoustic concentrating along with a higher-velocity digital camera combine to picture these T cells constantly at minimal or high flow costs. Effortlessly alter focus and digicam options to meet experimental demands.

The Attune NxT cytometer would be the dependable workhorse instrument your lab can rely upon for regular and trustworthy performance. check here The Attune CytPix design features a large-velocity brightfield camera and AI-pushed picture Investigation program, with modern upgrades which include device Studying applications and an intuitive user interface, enabling Improved data precision, accuracy, and speedier insights into sample biology.

Maybe most importantly, these situations incorporate cells of Plainly diverse phenotypes which may lead to incorrect conclusions about double optimistic gatherings (especially in rare populations).

Seem waves help specific mobile alignment, leading to Fantastic data quality and ~10X more rapidly sample flow costs.

We previously demonstrated the strength of imaging Automobile-T/Ramos mobile interactions. Allow’s look at just the inhabitants of biggest curiosity, the double beneficial situations, to learn more. We will now use prolonged morphological parameters (here, circularity and skewness of intensity) to even more study the attributes of these populations and refine gating on these events, increasing information robustness.

Leverage the strength of advanced technologies and innovation to accelerate your discoveries. Have faith in from the Attune Flow Cytometer loved ones to provide the dependability, velocity, and accuracy that set your do the job aside.

No wash/no lyse leukocyte Investigation with imaging. Cells were being acquired from 24-hour-old blood by dilution in one mM EDTA ( Pro UX/UI permits end users to teach AI styles for expanded apps. (A) The “Train Model” aspect presents annotation instruments and true-time model training precision.

At the center of each and every Attune Flow Cytometer lies our groundbreaking acoustic focusing technologies, which utilizes audio waves to meticulously align cells inside the flow mobile. This modern solution surpasses regular hydrodynamic solutions, resulting in more quickly processing speeds, exceptional data good quality, and amplified sample throughput.

The Attune Xenith cytometer can be a high-parameter spectral analyzer, furnishing each spectral unmixing and conventional flow capabilities for comprehensive and functional cell Investigation.

Agilent Value Guarantee ensures you a minimum of a decade of instrument use within the day you purchase. Usually, we will credit rating you While using the residual price of the method toward an upgraded design.

In this article, we demonstrate that by gating techniques using graphic-primarily based quantitative parameters can distinguish interacting cells from coincident functions to additional properly assess interacting cells.